Human iPSC provides an attractive tool for studying early eye development and the pathogenesis of eye diseases. Creative Bioarray is committed to helping clients build iPSC-based models of eye diseases to provide an effective platform for understanding the pathophysiology of multiple eye diseases and identifying molecular therapeutic targets for clinical applications.
iPSC for Modeling Eye Diseases
The pathogenesis of eye diseases is complex and the eventual loss of vision negatively affects patients in many ways, including quality of life, mental health, educational opportunities, and independence. In numerous reports, scientists have made considerable progress in studying eye development and disease using animal models such as rats, zebrafish and Xenopus. However, the fact is that animal models are not optimal. Currently, the use of iPSCs for disease modeling has become an attractive option.
In disease modeling, patient-derived iPSCs can be combined with 3D culture for organoid differentiation, which helps to further analyze the contained cell types and the interactions between different cell types. Many methods of organoid differentiation have been reported, including retinal organoids, liver organoids, and brain organoids. In addition to 3D culture, researchers have also established disease models of different retinal cell types to provide a valuable platform for studying the pathogenesis of various eye diseases.
Fig.1 Comparison of the morphology of retinal pigment epithelial stem cells derived from human embryonic stem cells (HESC), post-mortem (PM) eyes and iPS cells. (Chen, 2014)
Our Solutions
Researchers at Creative Bioarray have successfully established disease models for different retinal cell types, including retinal pigment epithelium (RPE), retinal ganglion cells (RGCs), and photoreceptor cells. To help our clients better understand the pathophysiology of many eye diseases, we offer the following solutions:
- Developing iPSCs-based primary open-angle glaucoma (POAG) models.
- Developing iPSCs-based age-related macular degeneration (AMD) model.
- Developing iPSCs-based retinitis pigmentosa (RP) model.
- Developing iPSCs-based developmental eye disorders models including microphthalmia and corneal hereditary endothelial dystrophy.
Applications
- Testing gene therapy
- Drug screening
- Development of cellular therapies for developmental eye disorders and degenerative diseases of the retina
Advantages
- Many years of experience in disease modeling
- Advanced cell reprogramming techniques and gene editing technologies
- Customization and optimization of experimental conditions according to customer requirements
- Delivery of detailed and complete experimental reports
As a leading service company in the field of cell reprogramming, Creative Bioarray provides advanced technologies to help clients establish iPSC-based models of eye diseases. We would be glad to discuss the details of improvements in reprogramming methods to develop the best solution for you. Please contact us for technical support.
Reference
- Chen, F.K.; et al. iPS cells for modelling and treatment of retinal diseases. Journal of clinical medicine. 2014, 3(4):1511-1541..