Neuron-Glial 2 (NG2) cells have received attention from researchers as promising cellular substrates. Creative Bioarray is focused on developing efficient new methods to provide clients with services to reprogram NG2 cells into induced neurons. Our expert technical support will help you explore ways to generate new neurons in situ for brain repair.
NG2-to-Neuron Reprogramming
The development of direct reprogramming techniques has opened new prospects for generating neurons for transplant-based cell replacement therapies. To date, various somatic cell types, including pericytes, fibroblasts, and glia, have been successfully reprogrammed into neurons. NG2 cells, also known as oligodendrocyte progenitor cells or glial progenitor cells, are receiving increasing attention from neuroscientists for their abundance and proliferative capacity. Because NG2 cells can maintain their numbers after depletion, this makes them a promising source of donor cells and an excellent target for regenerative medicine.
Currently, it has been proposed that after brain injury in mice, reactive neuroglia including NG2 cells and astrocytes can be directly reprogrammed into functional neurons through infection of retroviral encoding a single TF, NeuroD1. Preliminary studies to obtain direct neuronal reprogramming of NG2 cells in mouse models in vivo have laid the foundation for research on human cells for transformation. Recently, a regenerative and reproducible stem cell-based human NG2 cells system has been established to directly reprogram human fetal- and stem cell-derived NG2 cells into midbrain dopaminergic neurons.
Fig.1 Sox2-mediated transformation of NG2 cells into inducible neurons in the injured adult cerebral cortex. (Heinrich, 2014)
Our Technical Support
In order to provide our clients with services to reprogram NG2 cells into induced neurons, we have developed several effective and rapid strategies by using different combinations of TFs. The technical support we provide includes but is not limited to:
- Direct reprogramming of NG2 cells into induced dopaminergic neurons for producing a treatment-related cell type that selectively degenerates in Parkinson's disease.
- Direct reprogramming of NG2 cells into neurons with fast-spiking parvalbumin-containing interneuron properties.
- Direct reprogramming of NG2 cells into GABAergic inhibitory neurons.
- Direct reprogramming of NG2 Cells into Inhibitory interneurons.
Applications
- Generation of patient- and disease-specific neurons
- Study on disease modeling and diagnostics
- Biomedical applications for brain repair
Advantages
- Formulation of the most suitable solutions
- Reasonable price and fast turnaround time
- Reliable experimental report with timely update
With our extensive expertise in the field of cell biology, Creative Bioarray provides our customers with high-quality neural reprogramming services. We look forward to being your scientific assistant and providing you with first-class technical support. For more information about our services for reprogramming NG2 cells into neurons, please contact us for inquiries.
Reference
- Heinrich, C.; et al. Sox2-mediated conversion of NG2 glia into induced neurons in the injured adult cerebral cortex. Stem cell reports. 2014, 3(6): 1000-1014.