As a leading biotechnology company in the field of cell reprogramming, Creative Bioarray is committed to providing our customers with satisfactory cell reprogramming services. We focus on extracellular forces including shear stress, mechanical stretch and electromagnetic forces to explore new strategies for improvement of cell reprogramming efficiency.
Introduction
Extracellular mechanical forces can ultimately have an impact on organ development and function by initiating a series of cytoskeleton-mediated mechanotransduction and intracellular signaling cascades. Currently, several studies have proposed that extracellular forces including mechanical stretch, shear stress and electromagnetic forces have profound effects on cell signaling and reprogramming.
- Mechanical stretch
- Fluid Shear Stress
- Electromagnetic forces
Mechanical stretch has been shown to have a positive effect on reprogramming. At the cellular level, the mechanical stretch has important regulatory roles on cell spreading, lineage commitment and stem cell differentiation on 2D matrices. The effect of mechanical stretching may rely on the specific signaling pathways involved in the reprogramming process. Moreover, various parameters of stretching, such as mode, rate, amplitude, frequency, duration and relaxation period, may also have different effects on the efficiency of reprogramming.
Fluid shear stress, an important mechanical factor in flowing fluids, can trigger mechanosensitive gene expression, induce cytoskeletal tissue remodeling, and alter cellular behavior. Therefore, dynamic suspension-induced reprogramming is a viable strategy in basic research and clinical applications.
Electromagnetic or electrical stimulation systems have the potential to act as electroceuticals to provide a non-chemical and less invasive approach to facilitate cell reprogramming in vivo or in situ direct cell transformation.
Fig.1 Electromagnetic fields mediate efficient cell reprogramming into a pluripotent state. (Baek, 2014)
Technical Support
The technical support we provide to our clients includes, but is not limited to:
- Inoculation of dermal fibroblasts transduced with reprogramming factors onto mechanically stretched flexible membranes and perform mechanical stretching to improve the yield of reprogramming.
- Faster and more efficient mouse iPSC reprogramming by stirred suspension bioreactors with retrovirally transduced transcription factors.
- We applied extremely low-frequency electromagnetic fields (EL-EMF) to significantly improve the efficiency of iPSC reprogramming in mice. In addition, we combined a triboelectric stimulation system with non-viral gene delivery to achieve more efficient reprogramming such as the direct transformation of fibroblasts into functional neural cells.
Our Advantages
- A dedicated research team consisting of industry-leading scientific experts
- Flexible and efficient solutions
- Detailed after-sales service and competitive prices
Creative Bioarray has taken the needs of customers in the field of cell reprogramming as the development direction of the company, and continuously expanded services and technologies to seek long-term development. We are well-positioned to help our clients develop strategies to improve the efficiency of cell reprogramming mediated by extracellular force stimulation. Please feel free to contact us if you have a need.
Reference
- Baek, S. et al. Electromagnetic fields mediate efficient cell reprogramming into a pluripotent state. Acs Nano. 2014, 8(10): 10125-10138.