Reprogramming B Cells into Macrophages

With a world-class technology platform, Creative Bioarray has developed an efficient strategy to provide customers worldwide with B cells reprogramming into macrophages. Our professional technical support will accelerate your research in the field of blood cell reprogramming.


Hematopoiesis is one of the classic systems for studying the mechanisms of lineage determination in vertebrates. Hematopoietic lineages are defined by a stepwise process of binary determination, starting with pluripotent progenitors that branch into a common lymphoid progenitor and a common myeloid progenitor, which then differentiate into other intermediate progenitors. Each lineage exhibits a unique pattern of gene expression, including a group of more than a dozen lineage-restricted transcription factors (TFs). Because random combinations of these factors could lead to multiple phenotypes, mechanisms must be in place to ensure highly coordinated control of TF network remodeling during commitment.

Unlike the early branching of lymphoid and myeloid compartments in adult bone marrow, the fetal liver possesses bipotent B cell/macrophage progenitors (B/M), indicating that the two lineages are closely related. Studies have also shown that some oncogene-immortalized B cell lines could be reprogrammed into macrophages through activated M-CSF receptor and raf/ras oncogenes. In both studies, although the frequency of cell conversion appeared to be low, the resulting bone marrow cells had the same immunoglobulin reconstitution as the original B lineage cells.

Fig 1. Effect of transient C/EBPα expression on OSKM-induced iPS cell reprogramming of B cells.Fig.1 Effect of transient C/EBPα expression on OSKM-induced iPS cell reprogramming of B cells. (Di Stefano, 2014)

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Fig 1. Effect of transient C/EBPα expression on OSKM-induced iPS cell reprogramming of B cells.

Expressing a given TF in cells where it is not normally present and determining whether these cells transdifferentiate into TF-related cell type could be used to detect the cell type instructive potential of the specific TF. Using this approach, Creative Bioarray reprograms normal B cells (including immunoglobulin expressing cells) by enforced expression of the TF C/EBPα to generate functional macrophages that retain immunoglobulin rearrangement. Where TF C/EBPα is expressed at high levels in macrophages and is necessary for the formation and function of these cells. We provide an indication for the development of clinical therapies by helping our clients to induce transdifferentiation.


  • Study on the pathogenesis of leukemia
  • Development of clinical therapies
  • Generation of a range of novel cells for conducting regenerative medicine research


  • Professional researchers
  • Advanced experimental platform
  • Efficient strategies
  • Reliable and considerate service

Creative Bioarray provides clients with C/EBPα-induced transdifferentiation of B cells to macrophages, which may be useful in the study of therapies for human diseases, such as the conversion of human B-cell lymphoma or leukemia cells into functional non-cancerous macrophages. If you have a need in this field, please contact us for consultation.


  1. Di Stefano, B.; et al. C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells. Nature. 2014, 506(7487): 235-239.
For Research Use Only. Not For Clinical Use.