Micro/nanotopography has been demonstrated to help determine cell fate. Researchers at Creative Bioarray are committed to helping our customers improve the efficiency of iPSCs reprogramming and direct reprogramming through micro/ nanotopography to meet their scientific needs. With our best-in-class cell reprogramming platform and extensive expertise, we can provide our clients with scientific services to their satisfaction.
Introduction
Topography has profound effects on cell functions including cell adhesion, proliferation, morphology, differentiation, migration, and stemness maintenance, ultimately helping to determine cell fate. Although numerous studies have shown that topography can guide cell differentiation into specific cell lineages, more recently an increasing number of studies have focused on the role of topography in cell reprogramming. The topological cues investigated range from the microscale to the nanoscale. Initially, microtopography was regarded as the focus of research as a means of reprogramming and differentiation, but in recent years nanotopography has received more attention due to increased similarity to the structure of the extracellular matrix in vivo.
It was proposed that biomaterial topography in the form of aligned nanofibers and parallel microgrooves significantly improved reprogramming efficiency by altering cell shape. Inhibition of DAC2 activity combined with upregulation of WDR5 expression leads to increased histone acetylation and methylation respectively, and enhanced mesenchymal-to-epithelial transition (MET). Subsequent studies have further highlighted the differential roles of nanofiber polymer properties in enhancing MET in human fibroblasts during reprogramming.
Fig.1 Topographical cues acting as micro-environments for cell adhesion and in vivo mimicry. (Long, 2017)
Technical Support
- Microtopography mediated reprogramming.
- Nanotopography mediated reprogramming.
We are committed to helping our customers improve the efficiency of cell reprogramming through microtopography according to their specific scientific needs, including iPSC reprogramming as well as direct reprogramming. For example, facilitating reprogramming of fibroblasts into induced neurons or improving the efficiency of direct cardiac reprogramming through topography modulation.
We mainly apply this technology to induce hiPSCs to differentiate into neuronal lineage because the nanoscale characteristics may more closely resemble the in vivo microenvironment. Our researchers developed strategies to use nanotopographical substrates as biomimetic tools to more efficiently study differences in disease models and cell differentiation.
Applications
- Improvement of the efficiency of cell reprogramming
- Study on cell behavior in a dynamic medium by tuning nanotopographical cues
- Development of drug screening and disease modeling
Our Advantages
- Good hardware facilities and rich expert resources
- Strong professionalism and efficient technical support
- Abundant experience in the field of cell reprogramming
Creative Bioarray focuses on the development of micro/nanotopography-mediated strategies to improve the efficiency of cell reprogramming to meet our clients' scientific needs. Our dedicated research team can provide efficient cell reprogramming services and the optimal solution to our clients. If you are interested, please contact us directly.
Reference
- Long, J.; et al. A biomaterial approach to cell reprogramming and differentiation. Journal of Materials Chemistry B. 2017, 5(13): 2375-2389.