With years of experience in the field of cell reprogramming,Creative Bioarray has developed a variety of efficient methods to provide high-quality karyotype analysis services for our clients, including examination of cell chromosome numbers and morphology.
Background
The accumulation of karyotypes during long-term iPSCs culture can lead to chromosomal abnormalities. Genomic alterations can result in changes in gene expression and cellular function of iPSCs, and even increase the risk of tumorigenesis. Therefore monitoring the genomic integrity of iPSC lines is critical in iPSCs used for therapeutic purposes.
The karyotypic analysis includes the examination of the number and morphology of cellular chromosomes, as well as monitoring changes in size, the position of mitoses and banding patterns, etc. Experts at Creative Bioarray recommend that karyotypic analysis of iPSCs lines should be performed every 10-15 generations to confirm that no chromosomal duplications, deletions, insertions, translocations, or loss of mitoses have occurred.
Fig.1 Characterization of iPS cells with abnormal karyotype. (Noto, 2014)
Karyotyping of iPSCs
Creative Bioarray has developed a variety of methods for iPSC karyotyping, including but not limited to:
- Comparative genomic hybridization analysis
- G-banding
- Multicolor FISH (M-FISH) analysis
- Molecular Karyotyping (CGH-array) Service
- Reverse transcription quantitative PCR (RT-qPCR) analysis
Comparative genomic hybridization is used to determine chromosome ploidy and test for copy number variations (CNV).
G-banding is a technique applied in the field of cytogenetics based on staining the chromosomes of medium-term cells with different banding patterns. This technique produces visible karyotypes via staining cohesive chromosomes and can be used to identify structural abnormalities.
M-FISH is used for precise chromosome separation and rapid chromosome classification, helping to decipher complex chromosomal rearrangements to accelerate the workflow. This method can detect intra- and inter-chromosomal translocations more accurately than traditional karyotyping.
CGH-array is a karyotype analysis method with an increased diagnostic yield for the detection of imbalanced numerical and structural chromosomal alterations with high-resolution and high throughput capabilities.
RT-qPCR provides a sensitive, rapid, and quantitative method for monitoring the gene expression profile of cell populations.
Features of Our Service
- High-resolution and high throughput
- High sensitivity and high accuracy
- Fast turnaround time and competitive prices
Creative Bioarray is committed to providing high-quality karyotyping services including traditional G-banding, M-FISH, CGH-array, and RT-qPCR to meet our clients' research needs and ultimately deliver a detailed scientific report with high-resolution images. Our professional technology platform allows us to provide the best research support in the field of karyotyping. If you need our services, please feel free to contact us.
Reference
- Noto, F.K.; et al. Aneuploidy is permissive for hepatocyte-like cell differentiation from human induced pluripotent stem cells. BMC research notes. 2014, 7(1): 1-9.