The technique of generating induced neural stem cells (iNSCs) by cell reprogramming has great potentials for cell replacement therapies and modeling of neurological diseases. Creative Bioarray is committed to developing multiple efficient strategies to help researchers reprogram skin cells into iNSCs and our scientific services will contribute greatly to the success of your research in related fields.
Introduction
Endogenous neurogenesis is not sufficient for replenishment and leads to the very limited self-repair of neurodegenerative diseases including Huntington's disease, Alzheimer's disease (AD), and glaucoma. NSCs have great potential to enhance the regeneration of damaged nervous systems and repair neurodegenerative diseases. Therefore, regenerative medicine is currently focusing on techniques to generate large numbers of neurons, glia, or their progenitor cells thus providing a promising approach for lesion repair. Somatic cell reprogramming has proven to be a valuable tool for deriving patient-specific NSCs.
In addition, engineered NSCs are considered promising in the treatment of glioblastoma (GBM), the most common primary brain tumor. Reprogramming a patient's own somatic cells to create human NSCs, called induced NSCs (h-iNSCs) has opened up the possibility of developing patient-specific therapies for central nervous system (CNS) diseases. Notably, transdifferentiation (TD) technology without passing through the undifferentiated pluripotent state is critically important for cell transplantation therapies and improves the transformation rates and efficiency as well as in vivo safety. TD cells are therefore ideal for cell replacement and they can avoid immunosurveillance since they are the patient's own cells.
Fig.1 Generation and characterization of diagnostic and therapeutic h-iNSCTE. (Bagó, 2017)
Our Strategies
After years of effort, Creative Bioarray has developed several safe and efficient strategies to provide researchers with the reprogramming of skin cells. Clients can choose the optimal strategy for their specific research needs.
- Direct reprogramming mouse and human fibroblasts into self-renewal iNSCs that can differentiate into functional neurons, oligodendrocytes and astrocytes through a single non-neural progenitor TF ptf1a or a single zinc-finger TF, zfp521.
- Induction of mouse fibroblasts into NSCs using only small molecules without the use of any exogenous TFs. This chemical induction method eliminates the risks associated with current techniques, such as the induction of oncogenic factors.
Applications
- Establishment of iNSCs for research and therapeutic purposes
- Development of cancer therapy
- Development of therapies for neurodegenerative diseases
Advantages
- Non-canonical and safer approaches
- Advanced and professional platform
- Concierge customer services
- Strict quality control
Creative Bioarray has extensive expertise and experience in the field of cell reprogramming. We have successfully reprogrammed mouse and human somatic cells into iNSCs by defined tissue-specific TFs or chemicals. The services we offer will accelerate your research. If you need related technical support, please contact us for specifics.
References
- Bagó, J.R.; et al. Tumor-homing cytotoxic human induced neural stem cells for cancer therapy. Science translational medicine. 2017, 9(375).
- Xiao, D.; et al. Direct reprogramming of fibroblasts into neural stem cells by single non-neural progenitor transcription factor Ptf1a. Nature communications. 2018, 9(1): 1-19.