Generation of Bovine iPSCs

Generation of Bovine iPSCs

Bona fide bovine iPSCs have great potential for biotechnology and agricultural applications. Creative Bioarray is committed to helping clients develop efficient strategies to successfully generate bovine iPS cell lines and provide extensive cell characterization services. We have the confidence to provide our clients with expert scientific services and accelerate their research in the field of cell reprogramming.

Bovine iPSCs

Domesticated cattle are of high economic value for meat and milk production. The availability of bovine PSCs would be useful to promote the application of advanced reproductive techniques in this species. Currently, genetic modification in cattle is achieved through somatic cloning using primarily fibroblasts. But the difficulty and relative inefficiency of gene targeting in somatic cells has limited the development of this approach. Recently, scientists have proposed that it is possible to generate iPSCs from cattle through similar approaches used for mouse or human cells, enabling the use of bovine pluripotent cells for basic or applied purposes. For example, bovine iPSCs with germline capabilities could be used to improve reproductive traits and biotechnology applications.

Since 2011, efforts and progress have been made in establishing bovine iPSCs. Many research groups have used OSKM or OSKM plus NANOG, or added large T antigen factors expressed in lentivirus, retrovirus, or piggyBac vectors to reprogram bovine fetal or adult cells. These cells showed some degree of pluripotency, but their reprogrammed state is incomplete. Overall, the establishment of fully reprogrammed, chimeric-compatible bovine iPSCs with long-term self-renewal capabilities remains a major challenge in the field and deserves further attention.

Generation of iPSCs from bovine embryonic fibroblast cells.Fig.1 Generation of iPSCs from bovine embryonic fibroblast cells. (Han, 2011)

Our Services

Researchers at Creative Bioarray focus on developing reproducible protocols to help clients reprogram bovine somatic cells into iPSCs, and we provide clients with the following services:

  • Establishing bovine iPSCs using defined transcription factors and modified culture media.
  • Developing new vector systems such as binary DNA transposon systems to mediate the reprogramming of bovine somatic cells into pluripotent iPSCs.
  • We provided cell characterization services, including karyotype analysis, immunofluorescence staining, real-time PCR analysis, and examination of the bovine iPSCs differentiation ability in vitro and in vivo.

Applications of Bovine iPSCs

  • Study on the embryonic differentiation in ruminants
  • Research on genetic modification of the bovine genome
  • Application in animal breeding
  • Development of veterinary and stem cell-based preclinical therapies in bovine disease models

Advantages of Our Services

  • Reliable scientific services
  • One-stop service from experimental design to data feedback
  • Short time and high efficiency

Equipped with professional researchers and a state-of-the-art cell reprogramming technology platform, Creative Bioarray provides efficient and reliable technical support to help our customers establish bovine iPSCs with long-term self-renewal capabilities. if you are interested in our services, please please contact us.


  1. Han, X.; et al. Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells. Cell research. 2011, 21(10): 1509-1512.
For Research Use Only. Not For Clinical Use.