Blood cell reprogramming may be a promising strategy for the use of transplantable stem cells for clinical applications. Creative Bioarray has committed to exploring efficient cell reprogramming strategies including the reprogramming of blood cells into hematopoietic stem cells (HSCs). Our expert technical support is critical for clients to gain insight into disease etiology through in vivo and in vitro disease modeling.
Introduction
HSCs are the only cells in the hematopoietic system that have the functional capacity to differentiate to all blood lines and to self-renew for life. These properties make HSCs promising in regenerative medicine. Allogeneic and autologous HSC transplantation are widely used clinically for the treatment of congenital and acquired hematopoietic disorders and other malignancies. However, allogeneic transplantation often results in graft versus host disease (GVHD), a devastating T-cell mediated disease. Isolation of homologous HSCs from patient-derived cells would avoid the inherent risks of HSC transplantation.
The generation of robust transplantable definitive HSCs from pluripotent cells currently remains elusive. Therefore it is necessary to find alternative strategies to pluripotent stem-cell-based approaches. It has been shown that ectopic expression of OCT4 combined with initiating signaling of hematopoietic cytokines can reprogram human fibroblasts into blood cell progenitors, but the resulting cells were unable to generate all blood cell lineages. Recently, an alternative strategy that has the potential to overcome this challenge has been proposed would be to reprogram differentiated blood cells to obtain inducible HSCs (iHSCs), capable of producing all cellular components of the blood like HSCs.
Fig.1 Graphical abstract of reprogramming committed murine blood cells to induced hematopoietic stem cells. (Riddell, 2014)
Our Strategies
Creative Bioarray has worked for many years to establish strategies to successfully reprogram blood cells into HSCs, and we offer our clients strategies that include but are not limited to:
- Activating the gene networks that control the HSC functional identity in committed blood cells by expressing a set of defined transcription factors. Moreover, the iHSCs derived under optimal conditions showed very similar gene expression characteristics to endogenous HSCs.
- Reprogramming of hematopoietic progenitor cells into HSCs with long-term transplantation reconstitution capacity by a set of chemical combinations.
Applications
- Studying the fundamental mechanisms underlying HSC identity
- Derivation of transplantable stem cells with clinical potential
- Advancing the development of regenerative medicine
Advantages
- World-class research platform
- Professional and efficient reprogramming strategies
- Research team of highly educated personnel
As a company highly specialized in cell reprogramming, Creative Bioarray has developed a wide range of reprogramming services to provide our clients with a cell-based platform for therapeutic screening. If you need services for reprogramming blood cells into HSCs, please contact us for more details.
Reference
- Riddell, J.; et al. Reprogramming committed murine blood cells to induced hematopoietic stem cells with defined factors. Cell. 2014, 157(3): 549-564.