Nuclear Transplantation Mediated Cell Reprogramming

As a biotechnology company focusing on the field of cell reprogramming, Creative Bioarray provides cell reprogramming technology mediated by nuclear transplantation to help researchers make research progress in many fields such as the preservation of endangered species, unique animal genotypes and transgenes.

Somatic Cell Nuclear Transfer

Somatic reprogramming refers to the process of inducing differentiated somatic cells to dedifferentiate into a pluripotent state with the characteristics of embryonic stem cells (ESCs). Somatic cell reprogramming can be realized by somatic cell nuclear transfer (SCNT), which is the main technology used in mammalian cloning. Therefore it has attracted much attention in the field of science and medicine. In 2013, American scientist Shoukhrat Mitalipov demonstrated that SCNT technology can produce blastocysts and be used to obtain human embryonic stem cells, setting the stage for nuclear transfer technology to enter the clinic as a therapeutic tool.

In addition to animal cloning, SCNT technology is also promising in stem cell biology and human therapeutics. The blastocysts generated by SCNT can be used to derive pluripotent stem cells (PSCs), also known as nuclear transplant ESCs (ntESCs), a derivation process termed therapeutic cloning. Since patient-derived ntESCs are genetically homologous to the donor patient, they are widely used for therapeutic purposes, including cell transplantation and disease modeling.

Fig 1. Diagram showing the major steps of reproductive and therapeutic cloning compared to natural fertilization.Fig.1 Diagram showing the major steps of reproductive and therapeutic cloning compared to natural fertilization. (Shogo, 2018)

Our Technology

The derivation of human ntESCs is challenging due to the low efficiency of cloned human embryos to reach the blastocyst stage. Over the years, Creative Bioarray has been committed to developing new methods to overcome the obstacles in SCNT technology. The cell programming technology platform we developed makes therapeutic cloning and reproductive cloning possible by increasing cloning efficiency.

  • We have developed a variety of strategies to help customers overcome the challenges in SCNT technology, including abnormal Xist activation hindering post implantation development, H3K9me3 hindering ZGA and preimplantation development, H3K27me3 imprinting defects hindering post implantation development, DNA methylation and other epigenetic obstacles.
  • We enhanced the development potential of SCNT by improving epigenetic reprogramming, including improving DNA methylation reprogramming, modifying histone markers, and strategies targeting X chromosome inactivation (XCI).

Applications of SCNT

  • Generation of novel human disease models for drug development
  • Development of therapeutic cloning and reproductive cloning
  • Rescuing near-extinct animals without sacrificing donor animals
  • Drug screening and evaluation

Advantages of Us

  • Fast reprogramming strategy, chromatin accessibility and transcriptome reprogramming within hours
  • More efficient reprogramming strategies
  • Real-time guidance from professional scientists

Creative Bioarray is committed to providing our customers with first-class cell reprogramming technology. The SCNT reprogramming technology we provide will accelerate your research progress. If you have interesting in our cell reprogramming platform, please contact us for more detailed information.


  1. Shogo, M.; Zhang, Y. Somatic cell nuclear transfer reprogramming: mechanisms and applications. Cell stem cell. 2018, 23(4): 471-485.
For Research Use Only. Not For Clinical Use.