In vivo neural reprogramming technology offers a powerful and promising tool for regenerative medicine research. Creative Bioarray has established multiple methods to provide clients with direct reprogramming of local glial cells into neurons to replace neurons in vivo. Our dedicated research team will provide you with the best solution to accelerate your research.
Glia-to-Neuron Reprogramming
Neurodegenerative diseases and acute brain injuries are often accompanied by massive neuronal loss. However, the adult mammalian brain has a very limited ability to regenerate in the presence of neural injuries or degeneration. In order to develop effective therapies, scientists need to overcome the major challenge of promoting neural regeneration for central nervous system repair under various pathological conditions. In recent years, researchers have focused on two promising approaches including the use of exogenous or endogenous cell sources in the field of neuronal replacement therapies and regenerative medicine. The latter involves reprogramming local non-neuronal cells such as glial cells into neurons through the introduction of neurogenic factors.
Considerable progress has been made in glial-to-neuronal reprogramming in vivo since the first report of neuronal repair by cellular reprogramming techniques after brain injury in mice in 2005. For example, expression of a pro-neuronal factor Ngn2 driven by retroviral vectors in combination with a survival factor Bcl2 led to a significant improvement in neuronal reprogramming after cortical injury in mice. Various reports on the optimization of reprogramming protocols establish neuronal reprogramming as a promising strategy for future application in the treatment of neuronal loss.
Fig.1 Innate neurogenesis and induced neurogenesis in the adult central nervous system. (Tai, 2020)
Our Strategies
In the past ten years, we have continuously improved reprogramming schemes, such as using better-performing neurogenic factors to gradually improve the efficiency of reprogramming. The strategies we offer include but are not limited to:
- Reprogramming of glial cells by SOX2-mediated in vivo reprogramming strategy, which undergoes a scalable neural progenitor phase that is able to generate various neurons from single reprogrammed glia.
- Direct conversion of glial cells into neurons through ectopic expression of transcription factors that play critical functions in normal neurogenesis without the need to pass through an expandable progenitor cell stage in the adult brain after injury.
Our Services
- Reprogramming Astrocytes into Neurons
- Reprogramming NG2 Cells into Neurons
- Reprogramming Olfactory Ensheathing Cells into Neurons
Applications
- Induced neurons after brain injury
- Induced neurons for Alzheimer's disease
- Induced neurons for Parkinson's disease
- Induced neurons after spinal cord injury
Advantages
- State-of-the-art facilities
- Efficient communication
- Abundant expert resources
- Custom services
Creative Bioarray offers comprehensive and flexible neuroglial cell reprogramming services, and our innovative technology combined with a dedicated research team gives us the confidence to handle your project and save you valuable time and effort. If you need further details, please feel free to contact us.
Reference
- Tai, W.; et al. Regeneration through in vivo cell fate reprogramming for neural repair. Frontiers in cellular neuroscience. 2020, 14: 107.