Blood cell reprogramming holds broad promise for future disease modeling and therapeutic development. Creative Bioarray has focused for many years on generating iPSCs from blood cells using a variety of cell reprogramming strategies to meet the scientific needs of our customers. We have the confidence to provide you with professional and efficient services to accelerate your research in the field of cell reprogramming.
Blood Cell Reprogramming to iPSCs
With the development of cell reprogramming technology, the types of cells that can be reprogrammed have expanded to more readily available cell sources, such as hair, wisdom teeth, urine, and blood cells. Among these, the technology for the generation of iPSCs from haematopoietic lineages has matured and developed over the years. The first report of hiPSCs derived from blood was reported in 2009, using retroviral transduction of reprogramming factors OSKM. This proof-of-principle experiment showed that human hematopoietic lineage has the potential to be transformed into a pluripotent state. Subsequently, an increasing number of researchers have followed up to explore such potential.
CD34+ hematopoietic cells and terminally differentiated blood mononuclear cells are the most commonly used cell sources for blood reprogramming. It has been reported that reprogramming different types of blood cells with multiple methods can successfully generate iPSCs. Moreover, iPSCs reprogrammed from blood cells display typical pluripotent phenotypes, including demethylated OCT4 or NANOG regulatory regions, pluripotency gene expression, the ability to differentiate into specific lineages in vitro, and the ability to form teratomas when transplanted into immunodeficient mice. The time required for the appearance of iPSC-like colonies after transduction or transfection usually depends on the cell sources and strategies used.
Fig.1 Generation of hiPSCs from peripheral blood. (Li, 2016)
Our Services
Over the past 10 years, Creative Bioarray has focused on developing services in the field of blood reprogramming and has matured technically. Blood cells at different stages of differentiation are a good starting source for reprogramming, and we offer our clients a variety of strategies including integrative and non-integrative strategies to reprogram different types of blood cells into iPSCs.
Applications
- Establishment of disease models of various blood diseases
- Study on the pathogenesis of myeloproliferative disorders
- Development of a new treatment for severe congenital neutropenia
Advantages
- Safe and efficient
- Easy to access, no harm to patients
- Stable and fast
- Reliable after-sales services
As a biotech company focused on cell reprogramming, Creative Bioarray is committed to continuously improving the establishment of blood cell reprogramming platforms and helping researchers further investigate the molecular signaling pathways of blood cell dedifferentiation to elucidate the mechanisms of blood cell reprogramming. If you are interested in the services we provide, please contact us directly.
Reference
- Li, Y.; et al. Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair. Stem Cell Research & Therapy. 2016, 7(1): 1-11.