The emergence of cellular reprogramming technology offers a new approach to the treatment of blinding retinal diseases. Creative Bioarray is committed to developing multiple reprogramming strategies to provide our clients with services to convert Müller glial cells into photoreceptors. Our professional scientific services will accelerate your research progress in the field of neural reprogramming.
Background
The loss of photoreceptors in multiple degenerative eye diseases is a major cause of visual impairments and blindness. Photoreceptors are a special type of light-sensitive neuron that enables vision. The adult mammalian retina has the little regenerative capacity, resulting in the inability of their nervous system to replace neurons lost due to degeneration. Therefore the development of effective methods for photoreceptor regeneration holds the very real prospect for alleviating ocular blindness in patients.
Müller glial cells are the major glial cell type in the retina, a component of the mammalian central nervous system that typically helps maintain retinal homeostasis and integrity. In mammals, Müller glial cells typically respond to retinal injury by becoming reactive and undergoing glial proliferation. Studies have now reported therapeutic approaches to reprogram Müller glial cells as replacement retinal neurons through specific factors expression, making pioneering observations for the development of potential strategies to generate photoreceptors through cellular reprogramming techniques. In conclusion, future applications of in vivo reprogramming offer an exciting regenerative method to replace photoreceptor losses in retinal diseases.
Fig.1 Transplanted HSPCs fuse with MG upon photoreceptor damage. (Sanges, 2016)
Our Technical Support
To meet the scientific needs of our clients, we have developed a variety of reprogramming techniques to provide our clients with services to convert Müller glial cells into photoreceptors. We provide customers with the following technical support:
- We help our customers screen and identify the optimal mixture of reprogramming factors that allow in vitro reprogramming of human Müller glial cells into induced photoreceptors using the CRISPR activation (CRISPRa) system.
- We provide in vivo cell fusion technology to reprogram Müller glial cells into retinal precursors that then differentiate into photoreceptors.
- We provide qPCR, immunocytochemistry, and single cell transcriptome analysis for photoreceptor marker analysis in reprogrammed photoreceptors.
Applications
- Study on molecular biological mechanism of neural cell differentiation induced by proneural gene
- Development of strategies for rescuing retinal degeneration phenotypes
- Research on personalized disease modeling
Advantages
- Reliable and professional scientific services
- Short experimental cycle and stable experimental system
- One-stop service
Creative Bioarray offers you a full range of scientific services. Our professional and innovative research team will design and modify cell reprogramming strategies to obtain photoreceptors according to your scientific needs. If you need our technical support, please contact us for consultation.
Reference
- Sanges, D.; et al. Reprogramming Müller glia via in vivo cell fusion regenerates murine photoreceptors. The Journal of clinical investigation. 2016, 126(8): 3104-3116.