iPSCs Characterization Services

iPSCs Characterization

iPSCs Characterization Services

With the rapid development of cell reprogramming, the generation of iPSC has become increasingly easier. However, a variety of factors including cell origin, derivation, passaging and culture conditions can affect iPSC properties. Therefore, we still need a deeper understanding of iPSC and the process of reprogramming and differentiation in vitro. At present, reprogramming human iPSCs from somatic cells has become more and more mainstream in basic and applied research. It is necessary to perform rigorous molecular and functional analysis to evaluate the pluripotency of iPSCs and eliminate genetic aberrations caused by reprogramming and in vitro culture pressure in iPSC lines.

Creative Bioarray uses a defined class of tests to characterize multiple features of iPSCs such as cell and colony morphology, growth rate, expression of surface markers, expression of pluripotency marker genes, methylation status, and differentiation into somatic cell types in vitro and in vivo. Rigorous characterization of pluripotency markers and function helps to confirm that iPSCs have fully met regulatory requirements to provide reliable scientific data and models for preclinical studies for clinical use.

Fig 1. Generation, expansion, and characterization of human iPSCs.Fig.1 Generation, expansion, and characterization of human iPSCs. (Baghbaderani, 2016)

iPSCs Characterization Services

To assess the pluripotency, safety and confirm the identity of iPSCs, we have developed multiple viable and cost-effective characterization methods based on our well-established and state-of-the-art cell reprogramming platform. The iPSC characterization services we offer include but are not limited to:

  • Pluripotency Marker Assays for iPSC
  • We assess the pluripotency potential of iPSC by measuring the expression of alkaline phosphatase (AP) associated with pluripotency and iPSC markers.

  • Karyotype Analysis for iPSC
  • We provide high-quality karyotyping services, including traditional G-banding, multicolor FISH (M-FISH) analysis, molecular Karyotyping (CGH-array) and RT-qPCR.

  • Differentiation Potential Analysis for iPSC
  • We provide efficient strategies including teratoma formation assays, embryoid body (EB) formation, and assessment of the potential for directed differentiation into specific lineages to predict the differentiation potential of iPSC.

  • Genome Sequencing Analysis for iPSC
  • We have developed some effective strategies to provide high-quality iPSC genome sequencing services to our clients.

  • Converted Cell Characterization Services
  • We provide electrophysiological characterization of iPSC-derived cell lines, cell function evaluation, and have developed new analytical methods for analyzing the characteristics of converted cells.


  • High sensitivity and high specificity
  • High accuracy and stability
  • Short turnaround time and competitive pricing

Creative Bioarray is an experienced provider of cell reprogramming services. We are committed to providing customers with comprehensive iPSC characterization services and detailed data for their scientific research. Based on our advanced cell reprogramming platform, we have confidence to provide the best service to meet each of our clients' needs. Please contact us if you need scientific services for iPSC characterization. We look forward to contributing to your project in the future.


  1. Baghbaderani, B.A. et al. Detailed characterization of human induced pluripotent stem cells manufactured for therapeutic applications. Stem Cell Reviews and Reports. 2016, 12(4): 394-420.
For Research Use Only. Not For Clinical Use.