With years of exploration in the development of cell reprogramming technologies, Creative Bioarray is committed to providing a full range of cell reprogramming services to customers worldwide. We help our clients develop nanocarriers or nanoparticles-based chemical delivery methods to improve reprogramming efficiency and safety. Our first-class reprogramming platform and the skillful scientific team will accelerate your research in the field of cell reprogramming.
Background
In recent years, nanocarriers- or nanoparticles-based chemical delivery methods have gained increasing interest and popularity in the biomedical and gene delivery fields due to multiple advantages including the large surface area for loading molecular cargo, suitable size for cell penetration, and protection of the loaded materials. Although non-viral gene delivery vectors may be less efficient than virus-based methods, nanoparticles are often considered conducive to viral transduction because of their larger transgene delivery, ease of transfection, low immunogenicity, low gene integration, and minimal risk of insertional mutations.
Currently, chemical-based nanovectors, including polymers, lipid, and hybrid systems, have been widely used to deliver plasmid DNA, mRNA, miRNA, recombinant proteins and small molecules for the development of therapies for a variety of diseases and have been applied to deliver reprogramming factors for cell reprogramming.
Fig.1 Generation of iPSCs through transfection via CPEPS-OS-miR nanoparticles. (Deng, 2015)
Chemical Delivery Systems
- Lipid nanoparticles
- Polymer nanoparticles
- Inorganic/hybrid nanoparticles
We have developed lipid-based vectors for efficient in vivo and in vitro cell reprogramming, such as reprogramming human fibroblasts into iPSCs by using cationic lipid vectors for delivery of modified RNA mixtures.
We have developed polysaccharide nanoparticles and several synthetic polymer nanoparticles such as poly (β-amino ester), cationic bolaamphile, and poly-(amidoamine) dendrimer for the delivery of reprogramming factors. This strategy offers the potential to control nucleic acid transfection in vivo and in vitro, providing the possibility and flexibility to manipulate cell fate.
We have developed inorganic and hybrid particles including mesoporous silica, graphene oxide, calcium phosphate, and gold-based nanoparticles for delivering reprogramming factors to enhance reprogramming performance.
Advantages
- No mutagenesis, no genetic integration, and no immunogenicity
- Applicable both in vitro and in vivo
- Diverse biomaterials are available
- Deliver diverse reprogramming factors without size limitations
Creative Bioarray is committed to providing high-quality cell reprogramming services to our customers. We have developed several chemical delivery systems including lipid nanoparticles, polymer nanoparticles, and inorganic/hybrid nanoparticles to help our customers improve reprogramming efficiency. If you are interested in our services, please contact us directly and send us your research needs, we have the confidence to provide you with the optimal solutions.
Reference
- Deng, W.; et al. MicroRNA replacing oncogenic Klf4 and c-Myc for generating iPS cells via cationized Pleurotus eryngii polysaccharide-based nanotransfection. ACS applied materials & interfaces. 2015, 7(34): 18957-18966.