Mechanical disruption is a cytosol delivery method that depends on rapid mechanical deformation of the cell resulting in transient membrane rupture. With a dedicated research team, Creative Bioarray is well-positioned to help customers develop the delivery systems for reprogramming factors based on mechanical disruption and provide reliable experimental protocols to provide customers with new ways in disease research and treatment.
Introduction
Mechanical disruption is a method of destroying cell membranes for transient transmission. Unlike electroporation techniques, mechanically triggered cell rupture is not relying on electric fields, endocytosis, exogenous materials, or chemical modification of target molecules. This approach is particularly suitable for applications involving proteins, nanomaterials, or cell types that are difficult to transfect.
Recent advances in microfluidics and nanotechnology have made mechanically disruption methods possible, such as cell squeezing and several nanostructure-based penetration methods. Although microfluidic and nanotechnology-based cell reprogramming delivery technologies are still in the early stages of rapid development, the emergence of these technologies have provided exciting delivery platforms for a wide range of delivery applications and hold great promise for providing different reprogramming factors for cell reprogramming in vitro and in vivo.
Fig.1 Mechanical disruption mediates cell reprogramming. (Sharei, 2013)
Technical Support
Combined with the progress in the field of microfluidics and nanotechnology, our researchers help customers develop mechanical destruction methods and apply them to the field of cell reprogramming.
- Cell squeezing
- Nanostructure-based cell reprogramming infiltration
We have developed a microfluidics-mediated cell squeezing method. Currently, we have successfully applied this technology to deliver OSKM recombinant proteins to human fibroblasts for iPSC reprogramming, and it has been proved that this method greatly improves colony formation.
We have developed nanostructure-based permeation methods including nanowires, nanoneedles, nanopears, and nanostraws. This strategy has the potential to deliver a variety of biomolecules into living cells in an efficient and high-throughput manner, and since nanoscale devices can penetrate the plasma membrane, we directly deliver different biomolecules (loaded on the surface or within the native structure) into the cytoplasm and nucleus.
Advantages of Our Services
- A professional and innovative research team
- Customer-specific and flexible solutions
- Reliable technical support and valuable technical guidance
- Extensive cell characterization services to ensure the effectiveness of delivery systems
With professional scientists devoted themselves to the field of cell reprogramming, Creative Bioarray is dedicated to improving the efficiency of cell reprogramming through the development of mechanically disrupted-mediated delivery systems. We look forward to working with you and providing scientific services to your satisfaction. Please contact us for more information.
Reference
- Sharei, A.; et al. A vector-free microfluidic platform for intracellular delivery. Proceedings of the National Academy of Sciences. 2013, 110(6): 2082-2087.